https://dspace.ctu.edu.vn/jspui/handle/123456789/63339 Fri, 13 Mar 2026 07:57:37 GMT 2026-03-13T07:57:37Z https://dspace.ctu.edu.vn/jspui/handle/123456789/71208 Title: Intravaginal administration of progesterone using a new technique for sustained drug release in goats Authors: Endo, Natsumi; Rahayu, Larasati Puji; Yamamura, Takashi; Tanaka, Hitoshi; Tanaka, Tomomi Abstract: The objective of the present study was to develop and evaluate a sustained release vaginal progesterone (P₄) capsule containing a mixture of mucoadhesive polymer and silicone fluid. Goats were administered a gelatin capsule containing 0.4 g of P₄ mixed in silicone fluid and either a hydroxypropylmethylccllulose (HM) or polyaclil starch (PA) base. The mean plasma P₄ concentrations at 2 and 12 h after administration were significantly higher in goats treated with PA capsules than in those with MM capsules. The plasma P₄ concentrations in goats treated with HM capsules increased and remained above 1.0 ng/ml for 96 h after administration, whereas the plasma P₄ concentrations in goats treated with PA capsules remained above 1.0 ng/ml for only 24 h after administration. In the next experiment, an HM capsule was attached to a silicone device and inserted in the vagina for 10 days. The plasma P₄ concentration remained similar to that of the natural luteal phase for 9 days. These results suggest that a mixture of mucoadhesive polymer and silicone fluid has the potential to be applied clinically as a sustained release base for estrus synchronization or hormonal therapy. Wed, 01 Jan 2020 00:00:00 GMT https://dspace.ctu.edu.vn/jspui/handle/123456789/71208 2020-01-01T00:00:00Z https://dspace.ctu.edu.vn/jspui/handle/123456789/71207 Title: Effects of twin pregnancy prevention strategies such as GnRH dose and drainage of the smaller follicle on ovulation in dairy cows with two follicles of pre-ovulatory size in the same ovary Authors: Garcia-Ispierto, Irina; López-Gatius, Fernando Abstract: We examined the effects of a single or 2.5-fold dose of dephereline [a gonadotropin-releasing hormone (GnRH) analogue] as well as the drainage of the smaller follicle at the time of insemination on ovulation in dairy cows with two follicles of pre-ovulatory size in the same ovary. The three study groups included 220 monovular cows (control), 110 non- drained cows with two follicles, and 110 cows with two follicles, of which one was drained. In each group, cows treated with a single dose or 2.5-fold dose of dephereline showed similar results following treatment. Ovulation failure of the non-drained follicle occurred in 29.1% of the drained cows, whereas ovulation occurred in 96% of the non-drained and control cows. Twin pregnancy was recorded in 19.4% of the pregnant non-drained cows with two follicles. In conclusion, the increased dephereline dose did not improve the ovulation rate in any group. Follicular drainage, however, prevented twin pregnancy in cows with two follicles, but also resulted in an increase in the non-drained follicle’s rate of ovulation failure. Wed, 01 Jan 2020 00:00:00 GMT https://dspace.ctu.edu.vn/jspui/handle/123456789/71207 2020-01-01T00:00:00Z https://dspace.ctu.edu.vn/jspui/handle/123456789/71206 Title: Iron deficiency induces female infertile in order to failure of follicular development in mice Authors: Tonai, Shingo; Kawabata, Akane; Nakanishi, Tomoya; Lee, Joo Yeon; Okamoto, Asako; Shimada, Masayuki; Yamashita, Yasuhisa Abstract: Iron is important for many cellular functions, including ATP synthesis and cell proliferation. Insufficient of iron in the diet causes iron deficiency anemia (IDA), which often occurs in people living in the world. Since 50% of women with IDA show amenorrhea, the relationship of between iron deficiency and reproductive function was assessed using mice fed a low Fe diet (LFD). The estrous cycle in the LFD mice was blocked at diestrus, which impair follicle development, and fertility. Wed, 01 Jan 2020 00:00:00 GMT https://dspace.ctu.edu.vn/jspui/handle/123456789/71206 2020-01-01T00:00:00Z https://dspace.ctu.edu.vn/jspui/handle/123456789/71205 Title: Production of genome-edited mice by visualization of nucleases introduced into the embryos using electroporation Authors: Wake, Yui; Kaneko, Takehito Abstract: Genome editing technology contributes to the quick and highly efficient production of genetically engineered animals. These animals are helpful in clarifying the mechanism of human disease. Recently, a new electroporation technique (TAKE: Technique for animal knockout system by electroporation) was developed to produce genome-edited animals by- introducing nucleases into intact embryos using electroporation instead of the microinjection method. The aim of this study was to increase the efficiency of production of genome-edited animals using the TAKE method. In the conventional protocol, it was difficult to confirm the introduction of nucleases into embryos and energization during operation. Using only embryos that introduced nucleases for embryo transfer, it will lead to increased efficiency in the production of genome-edited animals. Wed, 01 Jan 2020 00:00:00 GMT https://dspace.ctu.edu.vn/jspui/handle/123456789/71205 2020-01-01T00:00:00Z