Vui lòng dùng định danh này để trích dẫn hoặc liên kết đến tài liệu này: https://dspace.ctu.edu.vn/jspui/handle/123456789/104024
Nhan đề: Expression of global consensus non-structural protein 1 (NS1) of dengue virus serotypes 1 and 2 in Nicotiana benthamiana
Tác giả: Cu, Le Nguyen
Nguyen, Ngoc Luong
Tuong, Quoc Trieu
Nguyen, Hoang Loc
Từ khoá: Agrobacterium tumefaciens
Agroinfiltration
Consensus
Dengue virus
Non-structural protein 1
Serotype 1
Serotype 2
Năm xuất bản: 2023
Tùng thư/Số báo cáo: Vietnam journal of Biotechnology;Vol.21, No.03 .- P.513-529
Tóm tắt: Dengue viruses, which consist of four distinct serotypes, belong to the Flavivirus family, one of the most important arthropod-borne pathogen groups for humans. Vietnam is among the tropical countries that have endemic dengue outbreaks, and in 2022, the number of infections as well as fatal cases reached a record high, causing a great economic burden to the public health system. Although all four dengue serotypes have been circulating in Vietnam, serotypes 1 and 2 have been dominating in the last few decades. Since most dengue clinical complications result from secondary infection with a different serotype from the first infection, prior knowledge of the proportion of a population having seropositive to a certain serotype is very crucial for public health policy makers. The absence of comprehensive and regular serological epidemiological surveys in Vietnam has impeded progress in surveillance and the implementation of effective preventive measures. To facilitate research into dengue treatment and diagnosis, we created two synthetic, codon-optimized genes encoding for global consensus non-structural protein 1 (NS1) from dengue serotypes 1 and 2. We expressed these genes in Nicotiana benthamiana using the agroinfiltration technique. Western blot using Dengue patients’ sera indicated that the target proteins were expressed, likely in both mono and multimeric forms. Our results indicated that N. benthamiana is a good alternative to other methods of producing recombinant NS1.
Định danh: https://dspace.ctu.edu.vn/jspui/handle/123456789/104024
ISSN: 1811-4989
Bộ sưu tập: Công nghệ sinh học

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