Please use this identifier to cite or link to this item: https://dspace.ctu.edu.vn/jspui/handle/123456789/104024
Full metadata record
DC FieldValueLanguage
dc.contributor.authorCu, Le Nguyen-
dc.contributor.authorNguyen, Ngoc Luong-
dc.contributor.authorTuong, Quoc Trieu-
dc.contributor.authorNguyen, Hoang Loc-
dc.date.accessioned2024-06-27T12:58:20Z-
dc.date.available2024-06-27T12:58:20Z-
dc.date.issued2023-
dc.identifier.issn1811-4989-
dc.identifier.urihttps://dspace.ctu.edu.vn/jspui/handle/123456789/104024-
dc.description.abstractDengue viruses, which consist of four distinct serotypes, belong to the Flavivirus family, one of the most important arthropod-borne pathogen groups for humans. Vietnam is among the tropical countries that have endemic dengue outbreaks, and in 2022, the number of infections as well as fatal cases reached a record high, causing a great economic burden to the public health system. Although all four dengue serotypes have been circulating in Vietnam, serotypes 1 and 2 have been dominating in the last few decades. Since most dengue clinical complications result from secondary infection with a different serotype from the first infection, prior knowledge of the proportion of a population having seropositive to a certain serotype is very crucial for public health policy makers. The absence of comprehensive and regular serological epidemiological surveys in Vietnam has impeded progress in surveillance and the implementation of effective preventive measures. To facilitate research into dengue treatment and diagnosis, we created two synthetic, codon-optimized genes encoding for global consensus non-structural protein 1 (NS1) from dengue serotypes 1 and 2. We expressed these genes in Nicotiana benthamiana using the agroinfiltration technique. Western blot using Dengue patients’ sera indicated that the target proteins were expressed, likely in both mono and multimeric forms. Our results indicated that N. benthamiana is a good alternative to other methods of producing recombinant NS1.vi_VN
dc.language.isoenvi_VN
dc.relation.ispartofseriesVietnam journal of Biotechnology;Vol.21, No.03 .- P.513-529-
dc.subjectAgrobacterium tumefaciensvi_VN
dc.subjectAgroinfiltrationvi_VN
dc.subjectConsensusvi_VN
dc.subjectDengue virusvi_VN
dc.subjectNon-structural protein 1vi_VN
dc.subjectSerotype 1vi_VN
dc.subjectSerotype 2vi_VN
dc.titleExpression of global consensus non-structural protein 1 (NS1) of dengue virus serotypes 1 and 2 in Nicotiana benthamianavi_VN
dc.typeArticlevi_VN
Appears in Collections:Công nghệ sinh học

Files in This Item:
File Description SizeFormat 
_file_
  Restricted Access
7.09 MBAdobe PDF
Your IP: 18.119.255.183


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.