Vui lòng dùng định danh này để trích dẫn hoặc liên kết đến tài liệu này:
https://dspace.ctu.edu.vn/jspui/handle/123456789/104072
Nhan đề: | Study on the transient expression of infectious bronchitis virus spike protein in Nicotiana benthamiana leaves |
Tác giả: | Nguyen, Thi Tra Ngo, Hong Duong Trinh, Thai Vy Le, Thi Tra My Nguyen, Thi Thu Hien Pham, Thi Van Doan, Thị Thanh Huong Pham, Bich Ngoc Chu, Hoang Ha Hoang, Thị Thu Hang |
Từ khoá: | Agroinfiltration Infectious bronchitis virus Nicotiana benthamiana Receptor binding domain S1 spike Vaccine |
Năm xuất bản: | 2023 |
Tùng thư/Số báo cáo: | Vietnam journal of Biotechnology;Vol.21, No.03 .- P.531-541 |
Tóm tắt: | Infectious bronchitis virus (IBV) is considered as one of the main causes of economic loss in chicken farms worldwide, especially in poultry producing nations. This virus, a member of the Coronaviridae family, is classified into different genotypes based on its surface spike glycoproteins which also play important roles in cell attachment and immune response. Due to continual genetic mutation of IBV, attenuated and inactivated vaccines show decreased effectiveness or lack of cross – protection; thus ongoing studies focus on the development of new generation vaccines to prevent new IBV outbreaks. This paper describes our study on expression of the antigenic region in S1 subunit of the spike in Nicotiana benthamiana. Two expression vectors carrying either S1 or receptor binding domain (RBD) coding gene (pCB301-S1 and pCB301-RBD) were constructed and transformed into tobacco leaves by agroinfiltration method. The RBD showed a clearly higher level of expression compared to the whole S1. Purification of RBD by immobilized metal ion chromatography and size exclusion chromatography represented a mixture of monomer, dimer and trimer glycoprotein with expected sizes in Western blot. In summary, this study demonstrated our primary success in establishing an expression model that could be used to investigate plant-based IBV recombinant vaccine. |
Định danh: | https://dspace.ctu.edu.vn/jspui/handle/123456789/104072 |
ISSN: | 1811-4989 |
Bộ sưu tập: | Công nghệ sinh học |
Các tập tin trong tài liệu này:
Tập tin | Mô tả | Kích thước | Định dạng | |
---|---|---|---|---|
_file_ Giới hạn truy cập | 4.77 MB | Adobe PDF | ||
Your IP: 18.227.209.89 |
Khi sử dụng các tài liệu trong Thư viện số phải tuân thủ Luật bản quyền.