Effect of lipid enhancement on biochemical and reproductive parameters of mudskipper (Pseudapocryptes elongatus, curvier 1816) broodstock via dietary lipid

Abstract

The research aims to determine the suitable lipid content in the diet for optimal growth,potential maturation, and biochemistry composition of the mudskipper Pseudapocryptes elongatus. The experiment are arranged according to 4 different treatments including the treatment lipid 6% is a control treatment; Lipid 9%; 12% and 15%. Added to the fish oil, plant oil-simply and ARA feeds corresponded to the treatments of the feed by mixing it and dried at 70°C for approximately 2 days and then stored in the fridge during the study. The results showed that the lipid content in the feed did not affect the survival rate of the mudskipper. The lipids in the feed composition affect the chemical composition in the muscles of the mudskipper. The lipid content in the liver of mudskipper is highest at 12% (76,13±0,51) and the lowest is the control treatment (73.96±.,45). The lipid content in the muscles of the mudskipper highest at 12% (3,47±0,20), the lowest in the treatment lipid 9% (2.28±0,39) and there was no significant difference (p>0,05). The HSI of mudskipper after 90 days of study ranged from 6.71-7.97%, the highest in the treatment 2: 9% (7.97±0.78).Lipids have an effect on the accumulation of energy and chemical composition in muscles and lipids in the liver of mudskipper. The highest GSI in this study was at 9% (0.35±0.21). From the results of the current study, the lipid content of 9% in the feed, it was enhancement reproduction of and lipid content of 12% in the feed, it was enhanced biochemical of mudskipper. The control treatment had the smallest egg size (average length 41,45±5,4 μm).All lipid supplementation treatments resulted in significantly larger egg sizes compared to the control. The results of histological observation show that the development stage of mudskipper is mainly detected in stage III and The treatment 15% lipid is a treatment yielded the largest eggs.