Please use this identifier to cite or link to this item:
https://dspace.ctu.edu.vn/jspui/handle/123456789/107755
Title: | Expression of transcription factors involved in epithelial-to-mesenchymal transition of the breast cancer cell line MCF-7 co-cultured with adipose tissue mesenchymal stem cells |
Authors: | Vu, Thi Tien Le, Hoang Duc Bui, Van Ngoc Nguyen, Trung Nam |
Keywords: | Gene expression Epithelial to mesenchymal transformation Co-culture Breast cancer Mesenchymal stem cells |
Issue Date: | 2023 |
Series/Report no.: | Vietnam journal of Biotechnology (Tạp chí Công nghệ sinh học);Vol.21, No.02 .- P.249-257 |
Abstract: | Breast cancer is the most frequently diagnosed cancer in women globally. The tumor microenvironment plays a vital role in epithelial-to-mesenchymal transition (EMT), leading to the invasion and metastasis of cancer cells. The tumor microenvironment includes all components of the tumor cells, including the extracellular matrix, tumor vasculature, mesenchymal stem cells, immune cells, and fibroblasts. Understanding the interactions between mesenchymal stem cells and cancer cells is essential in determining the role of mesenchymal stem cells in diagnosing and treating breast cancer. In this study, we present the result of co-culture between adipose tissue mesenchymal stem cells (ADMSCs) and breast cancer cells (MCF-7 cell line) and determine the expression levels of transcription factors involved in EMT, including Twist and Snail. The results showed that the proliferation of MCF-7 co-cultured with ADMSCs was not increased compared to MCF-7 mono-cultured. Determination of gene expression levels by qRT-PCR revealed a significant increase in the EMT-related transcription factors (Twist and Snail) in breast cancer cells upon co-culture with ADMSCs. There were also significant differences between the expression levels of IL-6 and AhR in MCF-7 cells co-cultured with ADMSCs and MCF-7 cells mono-cultured. The results suggested that ADMSCs promoted the EMT of MCF-7 cells, potentially via AhR/NF-κB pathways. |
URI: | https://dspace.ctu.edu.vn/jspui/handle/123456789/107755 |
ISSN: | 2815-5955 |
Appears in Collections: | Công nghệ sinh học |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
_file_ Restricted Access | 3.42 MB | Adobe PDF | ||
Your IP: 3.142.135.24 |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.