Please use this identifier to cite or link to this item: https://dspace.ctu.edu.vn/jspui/handle/123456789/107758
Title: Sequencing and analysis of the Meq oncogene of MDV causing marek’s disease in Bac Ninh province from 2019 to 2022
Authors: Doan, Thị Thanh Huong
Nguyen, Thi Thu Hien
Do, Thi Roan
Luu, Minh Duc
Le, Thi Hue
Le, Thanh Hoa
Le, Thi Kim Xuyen
Nguyen, Thi Khue
Keywords: Bac Ninh province
GaHV-2
Meq
Vietnam
MDV
Issue Date: 2023
Series/Report no.: Vietnam journal of Biotechnology (Tạp chí Công nghệ sinh học);Vol.21, No.02 .- P.281-291
Abstract: Marek’s disease is a contagious avian viral disease that is caused by a Marek’s disease virus serotype 1 (MDV-1) or gallid herpesvirus 2 (GaHV-2), a member of the genus Alphaherpesvirus, family Herpesviridae. Up to now, the disease has caused significant losses to the chicken farming industry in many provinces and cities across the country. In this study, four MDV-1 (GaHV-2) strains causing the disease in Bac Ninh province during the 2019-2022 period were identified by multiplex Polymerase Chain Reaction (PCR). Subsequently, the segments of 1020 bp encoding 339 amino acids of Meq gene (Marek’s disease virus EcoRI fragment Q) from four samples were collected and sequenced. The rate of identity and homology between the four Vietnamese strains and other global strains ranged from 89.4% to 100% and 87.6% to 100%, respectively. These rates were higher between the four Vietnamese strains and MDV-1 Chinese strains, ranging from 99.2% to 100% and 98.2% to 100%, respectively. The molecular characterization of the Meq gene revealed that all four of the GaHV-2 strains infecting chickens in the Bac Ninh province belonged to the highly virulent group, with a low proline ratio in Meq protein (ranging from 20.59-21.18%), consisting of three PPPP motifs and three interrupted motifs that contain mutations at the second position of the proline rich region (PRR): PPPP>P (Q/A/R)PP. Overall, this study provides valuable information on molecular characteristics of MDV-1 strains in Bac Ninh province during the 2019-2022 period.
URI: https://dspace.ctu.edu.vn/jspui/handle/123456789/107758
ISSN: 2815-5955
Appears in Collections:Công nghệ sinh học

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