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Title: | Bias distribution of Pristionchus nematodes found in Cat Tien and Cuc Phuong National Parks, Vietnam |
Authors: | Le, Tho Son Nguyen, Nhu Ngoc Do, Quang Trung |
Keywords: | Beetles Caenorhabditis nematodes Culture Isolation Pristionchus |
Issue Date: | 2023 |
Series/Report no.: | Vietnam journal of Biotechnology (Tạp chí Công nghệ sinh học Việt Nam);Vol.21, No.04 .- P.765-772 |
Abstract: | The Pristionchus nematodes are distributed in many places worldwide, so they should be diverse in many aspects of biology. The most updated species, P. pacificus, has many differences from and similarities to the standard model organism, Caenorhabditis elegans, and become a parallel but complementary laboratory model for research in genetics, diversity, and evolution. It therefore acquires the demonstration of wild-type isolates of the Pristionchus species in various habitats. In the survey of the soil nematodes, we collected vegetation samples from forests and used them to isolate nematodes. The nematodes were raised in the laboratory to determine their species. In this research, we report the finding of numerous strains within the genus Pristionchus. By comparing the 18S rDNA sequences as an advanced barcode for the classification of free-living nematodes, we identified them in two different species (P. pacificus and P. chinensis) and predicted one new Pristionchus species. Of them, P. pacificus strains are dominant, more in the north and less in the south of Vietnam, and they likely evolved independently over time. The P. chinensis and new Pristionchus sp. were less distributed and probably found by chance. This result extends the global diversity of the nematode genus Pristionchus, facilitating future studies of evolutionary nematodes. |
URI: | https://dspace.ctu.edu.vn/jspui/handle/123456789/107814 |
ISSN: | 2815-5955 |
Appears in Collections: | Công nghệ sinh học |
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