CLPC1 protein inhibition, antimycobacterial, and anti inflammatory properties of the metabolite from Streptomyces wuyuanensis collected in Nghe An province, Vietnam

Abstract

Streptomyces wuyuanensis A79 was isolated from the soil samples collected in Quynh Luong mangrove forest, Quynh Luu district, Nghe An province, Vietnam. This strain demonstrated antimycobacterial activity against Mycobacterium smegmatis. Compound 1 (indole-3-carboxylic acid) was isolated from the culture extract of S. wuyuanensis (A79). This compound displayed the affection of ATPase activity of the recombinant ClpC1 protein, a regulatory protein of Mycobacterium tuberculosis. In addition, compound 1 exhibited anti-inflammatory activity through the inhibition of TNF-α on LPS-stimulated macrophage RAW264.7. This is the first time compound 1 (indole-3-carboxylic acid) has been isolated from S. wuyuanensis. Indole-3-carboxylic acid was also reported for the first time its inhibition of M. tuberculosis ClpC1 protein.