Please use this identifier to cite or link to this item: https://dspace.ctu.edu.vn/jspui/handle/123456789/12530
Title: Assessment of the Genetic Variation of Malaysian Durian Varieties using Inter-Simple Sequence Repeat Markers and Chloroplast DNA Sequences
Authors: Siew, Ging Yang
Huỳnh, Kỳ
Tan, Soon Guan
Alitheen, Noorjahan Banu Mohammed
Salleh, Muhammad Fadzly
Ng, Wei Lun
Tan, Sheau Wei
Yeap, Swee Keong
Keywords: Chloroplast DNA sequence
DNA barcoding
Durio zibethinus
Genetic diversity
Inter- simple sequence repeat
Issue Date: 2018
Series/Report no.: Pertanika Journal of Tropical Agricultural Science;41 .- p. 321-332
Abstract: To date, 124 durian varieties have been registered with the Malaysian Department of Agriculture based on phenotypic characteristics. However, the levels and patterns of genetic variation among the varieties are still unknown. In this study, the leaves of 27 durian varieties were sampled from four durian orchards in Universiti Putra Malaysia, namely Bukit Ekspo, Putra Mart, Ladang Puchong and Ladang 5. Twenty five inter-simple sequence repeat (ISSR) primers were tested for PCR amplification on DNA samples. Twelve ISSR primers amplified 133 clear and reproducible DNA fragments and 122 (91.73%) were polymorphic, indicating a high level of genetic variation among these durian varieties. Primers flanking four chloroplast DNA (cpDNA) regions (trnL-trnF, atpB-rbcL and trnH-psbA intergenic spacers as well as the partial matK gene) were tested for PCR amplification. Two cpDNA regions (trnL-trnF and matK) were successfully amplified, but showed no variation in their DNA sequences, even when additional samples from Vietnam were included. The findings in this preliminary study lay a foundation for more comprehensive future studies on the genetic variation among durian varieties.
URI: http://dspace.ctu.edu.vn/jspui/handle/123456789/12530
ISSN: 1511-3701
Appears in Collections:Tạp chí quốc tế

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