Inhibitory Selaginellins from Selaginella tamariscina against LPS-induced NO production in RAW264.7 cells

Abstract

Three selaginellins have been isolated and structurally identified as selaginellin (1), selaginellin M (2), and selariscinin B (3) from Selaginella tamariscina, by interpretation of spectroscopic (¹H, ¹³C NMR and MS) data and comparing with literatures. Compounds 1-3 exhibited potential inhibition on lipopolysaccharide-induced nitric oxide (NO) production in RAW 264.7 cells with IC₅₀ values of 21.0±1.2, 4.5 ± 0.6, and 18.1±0.9 µM, respectively. Celastrol was used as positive control showing an IC₅₀ value of 1.0±0.1 µM. This obtained data reveals the potential of these selaginellin type compounds in the development of anti-inflammatory agents from medicinal plants.