In Vitro propagation of the new orchid Dendrobium trankimianum T. Yakawa

Abstract

Dendrobium trankimianum T. Yukawa is a beautiful, endemic orchid of Vietnam, a new species with a first published description in 2004. It is very rare and expected to be added to the IUCN Red List status - CR. In vitro studies of orchid D. trankimianum T. Yukawa were conducted in order to conserve and increase the genetic pool of this precious wild orchid species. The results showed that hill-strength MS medium supplemented with 2.0 tng/L BA and 0.5 mg/l. NAA (10.24 PLBs/explant; 90.11% explants former Pl.Bs) or lull-strength MS medium supplemented with 1.5 mg/L TDZ and 0.5 mg/L NAA (14.11 PLBs/explant: 92.06% explants formed PLBs) were the most suitable for protocorm formation.