Effect of plant growth regulators on growth and lipid accumulation of microalgal haematococcus pluvialis flotow in two-stage culture

Abstract

Haematococcus pluvialis cells were cultured in aerated liquid Bold’s Basal medium in two-stage (initial stage during in 7 weeks for increased biomass growth and second stage during in 3 weeks for increased lipid accumulation) with different volumes 250 mL. 10 L. and 1,000 L. With a volume of 250 ml, the medium was supplied with benzyl adenine (BA), indole-3-acetic acid (IAA) or gibberellic acid (GA₃) at concentration from 0.1 - 0.2 mg/L in initial stage and 1AA or GA₃ at conccntration troni 0.1 - 0.2 mg/L in second stage.