Screening of endophytes from rubber trees (hevea brasiliensis) for biological control of corticium salmonicolor

Abstract

28 leaves and living-tissue samples of rubber tree (Hevect brasiliensis) were collected from Ho Chi Minh City, Binh Fhuoc province and Binh Duong province (Viet Nam). We isolated and screened endophytes that have potential application as agents for biocontrol of Corticium salmonicolor, the agent of Pink Disease in rubber trees. As a result, 21 strains of endophytic bacteria and 14 strains of endophytic fungi were isolated. Antagonistic activity of the endophytes towards c. salmonicolor was checked by using a dual culture. Testing results showed that: T9, T15 and T16 strains have inhibited c. salmonicolor. T9 and T16 strains showed result that 100% of inhibiting c. salmonicolor at the concentration of 1:1. In the test of ability to kill c. salmonicoỉor, T9 and TI6 strains showed that they could kill c. salmonicolor after 3 sprays of bacterial filtrate. T9 and T6 strains, which were identified by biochemical methods, have similar characteristics to Bacillus thuringiensis.