Optimization of medium composition for enhancing chitinase production of Paecilomyces sp. P1 strain by using response surface methodology

Abstract

The fermentation medium of Paecilomyces sp. P1 was optimized to yield the maximual chitinase. In this study, we used the design of optimum multifactorial experiments Plackett-Burman to estimate effects factors on chitinase production. As the result, five factors: the concentration of chitin substrate, glucose, pepton, K₂HPO₄ and the ratio Ca²⁺/Mg²⁺ were the niost effective.