High-mannose type n-glycan binding specificity of a novel lectin from the red alga (betaphycus gelatinus)

Abstract

The red alga, Betaphycus gelatinus is one of carrageenan sources in the world. The lectin from the red alga B. gedatinus was isolated by a combination of aqucous cthanol extraction, ethanol precipitation, ion-exchange chromatography and gel filtration chromatography and was designated as BGI after the specific name of alga. Lectin gave a single band with molecular mass of about 19,000 Da in both non-reducing and reducing SDS-PAGE conditions, therefore lectin exists in monomer form.