Cloning of sucrose isomerase encoding gene from klebsiella singaporensis isb-36 and its expression in pichia pastoris

Abstract

Given potential health benefits including low glycemic Index, tooth friendly, suitable to infants, elderly and diabetic patients, isomaltulose was considered as a promising alternative sweetener to sucrose. Due to the presence of liposaccharide endotoxin in Serratia plymuthica CBS 574.44, a Gram-negative bacterium, and minute amount of formaldehyde carried over, purification of isomaltulose requires rigorous controls in industry.