Modified techniques in quantification of intracellular listeria monocytogenes in vitro infection

Abstract

The demand for reliablc melhods for the quantitation of intracellular bacteria is growing. Among modern methods such as PC'R and flow cytometry, traditional methods including colony forming unit assay and immune-fluorescence are still the two most commonly techniques worldwide. In colony forming unit assay, there are variations among publications, making data results inconsistent across studies. The aim of this paper is to evaluate available techniques and develop improved protocols for the quantification of intracellular Listeria monocytogenes (LM) in vi tro infection assay.