Synthensis and cytotoxicity of polyhydroxylated cholesterol derivatives

Abstract

Eight polyhydroxylated cholesterol derivatives (l-8) were prepared from cholesterol, using oxidative reagents as SeO2, OsO4/NMO, HCOOH/H2O2 and BH3/H2O2. Their structures were elucidated by using physical methods including NMR 1D and 2D. These compounds were evaluated against two cancer cell lines (Hep-G2, T98). Compounds 2, 4 and 8 inhibit human hepatocellular carcinoma cell line (Hep-G2) with IC50 values of 11.59, 11.89 and 6.87 µM' respectively. In addition, compound 8 exhibited strong cytotoxicity against T98 cell line (glioblastoma) with IC50: 2.28 µM.