Please use this identifier to cite or link to this item: https://dspace.ctu.edu.vn/jspui/handle/123456789/40849
Title: Release of chondroitin sulfate form the chicken keel cartilage by protamex hydrolysis
Authors: Nguyen, Thi Le Vien
Luu, Van Thien Phu
Nguyen, Thi Thanh Phuong
Tran, Thi Hong Hanh
Dong, Thi Anh Dao
Keywords: Glycosaminoglycans
Chondroitin sulfate
Protamex
Chicken keel cartilage
Hydrolysis
Issue Date: 2018
Series/Report no.: Vietnam Journal of Chemistry;Vol. 56, No. 05 .- P.596-601
Abstract: Glycosaminoglycan (GAGs) is one kind of mucopolysaccharide, the natural biocompound can be extracted from cartilage of sharks, pigs, cows, chickens, etc. It contains chondroitin-4-sulphate, chondroitin-6-sulphate, heparansulphate, dermatansulphate, keratan sulphate, and hyaluronic acid, links with protein chains and thus are called proteoglycan. Chondroitin-4-sulfate (CS), the highest proportion in GAGs of poultry cartilage, is using as a supplement of functional food for preventing and supporting treatment of arthritis and eye tissues. Therefore, it is necessary to release the CS from chicken cartilage that is abundant by-products of poultry processing. In this study, the hydrolysis conditions by protamex of chicken cartilage were investigated. The variables such as: pH, protamex content, incubation temperature, and hydrolysis time were optimized. The results of the analysis of variance (ANOVA) shown that the variables actively affected CS content. The optimal conditions of hydrolysis were pH of 5.81, ratio of enzyme per substances of 0.85 % W/Wprotein, temperature of 54.63°C and hydrolysis time of 204 minutes, GAGs content reached 25.28 %. Analysis by HPLC revealed CS content 17.69 % dry matter of raw material.
URI: https://dspace.ctu.edu.vn/jspui/handle/123456789/40849
ISSN: 2525-2321
Appears in Collections:Vietnam Journal of Chemistry

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