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https://dspace.ctu.edu.vn/jspui/handle/123456789/4243
Title: | Continuous production of pure maltodextrin from cyclodextrin using immobilized Pyrococcus furiosus thermostable amylase |
Authors: | Nguyễn, Phú Cường Nguyễn, Minh Thủy Park, Kwan-Hwa Park, Jong-Tae Kim, Do-Gyun Oh, Il-Nam Lee, Wang-Hee |
Keywords: | Pure maltodextrin Packed-bed reactor Continuous production Pyrococcus furiosus thermostable amylase Enzyme immobilization Cyclodextrin |
Issue Date: | 2016 |
Series/Report no.: | Process Biochemistry;51 .- p.282–287 |
Abstract: | Maltodextrin in specific length is of great interest because of its wide industrial applications. Previously, we have proven that Pyrococcus furiosus thermostable amylase (PFTA) produces high purity maltoheptaose (G7) from ┐-cyclodextrin (CD) at early reaction time. In this study, an optimal continuous process for pure maltodextrin production from CDs using PFTA with immobilization in a packed-bed reactor (PBR) was developed. For testing PBR, ┐-CD was used as a substrate to produce G7. The activity of immobilized PFTA in PBR retained 85% of the initial activity after 6 cycles, and the product purity was unchanged. The optimal conditions for the maximized G7-purity of 96.3% were predicted at 4.7 mL/min of flow, 1.1% substrate and 48.7 ◦C. Maltohexaose and maltooctaose, 92% and 97% purity respectively, were produced from CDs at the similar condition as well. Through this study, we successfully demonstrated the possibility of large-scale continuous production of pure maltodextrins from CDs. |
URI: | http://dspace.ctu.edu.vn/jspui/handle/123456789/4243 |
Appears in Collections: | Tạp chí quốc tế |
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