Five phenolic compounds from Marchantia polymorpha L. and their in vitro antibacterial, antioxidant and cytotoxic activities

Abstract

Lunularin (1), marchantin A (2), isoriccardin C (3), luteolin (4) and apigenin (5) were isolated from the ethyl acetate extract of Marchantia polymorpha collected at Lam Dong province, Vietnam. The chemical structures of these compounds were elucidated by HR-MS and NMR spectroscopic analysis as well as compared with data in the literature. The evaluation of the cytotoxic activity against MCF-7, Hep G2, NCI-H460, and HeLa cancer cell lines of the ethyl acetate extract and compounds (1) and (3) showed that (1) was potent against MCF-7 cancer cell with the IC₅₀ value of 4.59±0.38 µg/mL. The evaluation of DPPH radical scavenging activity displayed strong activities of the ethyl acetate extract, compounds (1) and (3) with SC₅₀ of 12.75±13.27, 3.99±0.47 and 12.81±0.43 µg/mL, respectively, while SC₅₀ of the positive control Trolox was 7.34±0.87 µg/inL. The antibactcrial evaluation on the ethyl acetate extract and two compounds (2) and (3) against staphylococcus epidermidis showed that (3) possessed remarkably antibacterial activity with the inhibition zone of 12.67 mm at a concentration of 50 µg/well.