Hepatoprotective effect ofsilymarin on chronic hepatotoxicity in mice inducedby carbon tetrachloride

Abstract

This study was designed to explore the ideal dose of CCI₄ for chronic hepatotoxicity in Swiss albino mice as well as to verify the effective dose of silymarin for protection. Five groups of mice were prepared, within them, group (I) was served as control. The animals of group (II), (III) and (IV) were treated with 10mL of CCl₄ solution (10, 20 and 30% CCU/olive oil, respectively)/kg b.w. for each three days, o.p. and during 6 weeks. Animals from group (V) were treated with silymarin by oral doses of 16mg/kg b.w. after one hour of every CCl₄ treatment as group (III). Serum alanine transaminase (ALT), total cholesterol (TC), liver weight/body weight ratio (L/B), liver tissue malondehydyde, protein carbonyls and histology properties were determined. The results showed that chronic hepatotoxicity induced by CCl₄ could be detected at CCl₄ concentration of 10mL CCl₄ solution (20% in olive oil)/Kg b.w. Beside, treatment with silymarin 16mg/Kg b.w. was verified as an effective dose for chronic hepatotoxicity protection.