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Title: | Three new coumarins from Saposhnikovia divaricata and their porcine epidemic diarrhea virus (PEDV) inhibitory activity |
Authors: | Yang, Jun Li Hà, Thị Kim Quy Oh, Won Keun Kim, Jinwoong Kim, Eunhee |
Keywords: | Saposhnikovia divaricata Coumarin glycosides Stereochemistry PEDV inhibitory activity |
Issue Date: | 2015 |
Series/Report no.: | Tetrahedron;71 .- p.4651-4658 |
Abstract: | Swine based agro-industries throughout the world are in big threat of new PEDV infection due to lack of efficient prophylactic defenses as well as dependable curing agents. Bioactivity-guided fractionation of a methanol soluble extract from radix of Saposhnikovia divaricata led to the isolation of three new (1-3) together with 10 known coumarins (4-13). The structures of new isolates (1-3) were established by extensive spectroscopic analysis and their absolute configurations were assigned based on ECD spectra calculation and analysis. Among all isolates, compound 5 revealed strongest inhibitory effect on PEDV replication. Quantitative real-time PCR data showed inhibitory effect of 5 on genes responsible for synthesis of PEDV vital structural proteins (GP6 nucleocapsid, GP2 spike, and GP5 membrane) in a dosedependent manner. Also, compound 5 demonstrated the inhibitory effect on PEDV GP6 nucleocapsid and GP2 spike protein synthesis as analyzed by western blotting. This study represents a new class of chemical entities for developing anti-PEDV agents. |
URI: | http://localhost:8080//jspui/handle/123456789/5047 |
Appears in Collections: | Tạp chí quốc tế |
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