Please use this identifier to cite or link to this item: https://dspace.ctu.edu.vn/jspui/handle/123456789/54564
Title: Định lượng domperidon trong huyết tương người bằng phương pháp sắc ký lỏng siêu hiệu năng ghép nối vói detector khối phổ (UPLC-MS/MS)
Authors: Tạ, Mạnh Hùng
Phạm, Thanh Huyền
Phan, Thị Nghĩa
Keywords: Domperidone
Determination
Human plasma
UPLC-MS/MS
Issue Date: 2018
Series/Report no.: Tạp chí Dược học;Số 507 .- Tr.29-34
Abstract: An ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was developed for determination of domperidone in human plasma with diphenhydramine as internal standard: Column - C18 (150 x 4.6mm, i.d., 5 pm); mobile phase - 0.05% formic acid - methanol - acetonitrile - isopropanol (suitable ratio): flow rate - 0,5 ml/min (isocratic elution). The UPLC-MS/MS was operated in the multi reaction-monitoring mode using the electrospray ionization technique. For injection samples, domperidone and the internal standard (diphenhydramine) were extracted from human plasma by liquid-liquid extraction with a mixture of diethylether and chloroform. Good linearity for standard curves was found at the range of 0.5 to 50 ng/mi for domperidone (mean correlation coefficient R² ≥ 0.99). The intra-day and inter-day precision and accuracy were within 88.8% - 106.8%. The method was excellently time-saving - the total run time was 4 min only. This method showed applicable to BA-BE studies of domperidone preparations.
URI: https://dspace.ctu.edu.vn/jspui/handle/123456789/54564
ISSN: 0866-7861
Appears in Collections:Dược học

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