Xây dựng phương pháp định lượng paracetamol trong viên nén bằng quang phổ Raman với mô hình phân tích hồi quy đa biến

Abstract

Paracetamol in tablets was quantitatively determined by Raman spectroscopy in combination with PLS (RS/PLS) and PCR (RS/PCR). 7 training batches of paracetamol tablets 0, 8, 28, 50, 70, 85, 100% were made to be subject to Raman spectral analysis; the observed data were then added Into the training dataset to build RS/PLS and RS/PCR models. Especially, the 70% verifying batches were made to get spectral data for the verifying dataset to verify the 2 above models. Both models showed selective, repeatable and accurate within certain acceptable limits, proved high potential to quantify QC. Besides, they were applied to quick-sifting and semi-quantitating content of paracetamol (as the main active principle) in 28 samples of 3 marketed tablet products. The contents and mean errors predicted by the two models for each of these batches were quite good, close to their individual label-average content. Moreover, individual paracetamol contents of the samples from the 3 above products were significantly different.