Tinh chế, xác định hàm lượng và đánh giá độc tính cấp của tác nhân kháng khuẩn linezolid

Abstract

Linezolid was synthesized, purified, and evaluated for acute toxicity. The purity of the synthesized linezolid was analyzed by HPLC as follows: Column - Phenomenex Rp18(5 pm, 150 mm x 4.6 mm); Flow rate -1.0 ml/min; Detector- UV (251 nm): Mobile phase - Monobasic ammonium phosphate buffer (0.23 g/1000 mL)/Methanol 75/25 (v/v). By calibration curves, the purity of the obtained linezolid was found to attain 99.7%, with two impurities in quite low percentages (0.012% and 0.021%). /4s thus, synthesized linezolid showed well meet the requirments by USP 2012. Acute toxicity of the linezolid in mouse was performed at the dose level of 5000 - 8000 mg/kgP of mouse and observed for 7 consecutive days. The LD^value (6.32 ± 0.24) g/1 kg of mouse proved low toxicity of linezolid.