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Title: | Xây dụng và thâm định quy trình định lượng pinoresinol diglucosid trong cao khô đỗ trọng bằng phương pháp HPLC |
Authors: | Trần, Thanh Tâm Nguyễn, Đức Hạnh Huỳnh, Trần Quốc Dũng Nguyễn, Đức Tuấn |
Keywords: | PinoresinoI diglucoside HPLC Eucommia ulmoides Oliv |
Issue Date: | 2018 |
Series/Report no.: | Tạp chí Dược học;Số 511 .- Tr.60-64 |
Abstract: | An HPLC for determination of the bioative phytochemical component named pinoresinol diglucoside (PDG) of Eucommia ulmoides Oliv., Eucommlaceae known as a biomarker for the plant spray-dried extracts. The procedure was optimized as: Column - Gemini NX C18 (250 x 4.6 mm; 5 pm), column temperature - 30°C; Detector - UV (228 nm); Flow rate -0.8 ml/min; Mobile phase - acetonitrile and water (in gradient mode, with acetonitrile (%) were 10, 18 %: 50 %; and 10 % at the corresponding time interval of 0-1,0; 1.0-17.0; 17.1-22 and 22.1-28 min. By validation, the proposed method proved the good compatibility, specificity and linearity (R² = 0.9994). The RSD values of the intra-day and inter-day precision were 0.28 % and 0.48 %, respectively, and the recovery percentages 98.07 - 98.35 %. In short, the proposed method met the requirements of validation and could be useful for establishing the quality standard of the E. ulmoides spray-dried extract and its related products. |
URI: | https://dspace.ctu.edu.vn/jspui/handle/123456789/54701 |
ISSN: | 0866-7861 |
Appears in Collections: | Dược học |
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