Xây dụng và thâm định quy trình định lượng pinoresinol diglucosid trong cao khô đỗ trọng bằng phương pháp HPLC

Abstract

An HPLC for determination of the bioative phytochemical component named pinoresinol diglucoside (PDG) of Eucommia ulmoides Oliv., Eucommlaceae known as a biomarker for the plant spray-dried extracts. The procedure was optimized as: Column - Gemini NX C18 (250 x 4.6 mm; 5 pm), column temperature - 30°C; Detector - UV (228 nm); Flow rate -0.8 ml/min; Mobile phase - acetonitrile and water (in gradient mode, with acetonitrile (%) were 10, 18 %: 50 %; and 10 % at the corresponding time interval of 0-1,0; 1.0-17.0; 17.1-22 and 22.1-28 min. By validation, the proposed method proved the good compatibility, specificity and linearity (R² = 0.9994). The RSD values of the intra-day and inter-day precision were 0.28 % and 0.48 %, respectively, and the recovery percentages 98.07 - 98.35 %. In short, the proposed method met the requirements of validation and could be useful for establishing the quality standard of the E. ulmoides spray-dried extract and its related products.