Please use this identifier to cite or link to this item: https://dspace.ctu.edu.vn/jspui/handle/123456789/54717
Title: Tổng hợp và xác định độ tinh khiết tạp chất liên quan D (ethyl 5-amino-1H-pyrazol-4-carboxylat) của alopurinol
Authors: Nguyễn, Trần Vân Anh
Lữ, Thiện Phúc
Phạm, Lê Ngọc Yến
Nguyễn, Đức Tuấn
Keywords: Allopurinol impurity D
Ethyl 5-amino-1H-pyrazole-4-carboxylate
Issue Date: 2018
Series/Report no.: Tạp chí Dược học;Số 512 .- Tr.32-36
Abstract: Allopurinol impurity D (ethyl 5-amino-1 H-pyrazole-4-carboxylate) was synthesized from ethyl (ethoxymethylene) cyanoacetate and hydrazine in the yield of 61.3 % (0.28 gram). By using HPLC - PDA, its chromatographic purity was determined over 99.0 %. As such, the obtained allopurinol impurity D was fairly satisfactory for establishment of the reference substance. Moreover, an HPLC method for purity determination of impurity D was developed as: column - XtimateR C18(5 µm; 250 x 4.6 mm); Detector- UV (247 nm; Mobile phase - methanol and water (40:60, v/v); Flow rate -1.0 mUmin. Validation in observance to ICH guidelines, the method showed to hand wide linearity range (25.16 - 503.10 µg/ml), high precision (RSD < 0.03 %). The method showed good selectivity, wide linearity range and high precision.
URI: https://dspace.ctu.edu.vn/jspui/handle/123456789/54717
ISSN: 0866-7861
Appears in Collections:Dược học

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