Xác định hàm lượng benzalkonium clorid trong chế phẩm dung dịch xịt mũi bằng kỹ thuật HPLC

Abstract

An HPLC method was developed for assay of benzalkonium chloride in nasal sprays, as: Column - Phenomenex C18 (250 x 4.6 mm, 5 pm); Column t° - 40°C; Detector - UV(210 nm); Mobile phase - a mixture of acetonitril and 10 mM solution of potassium dihydrogenphosphate pH 2.2 containing 0.3 % (v/v) of triethyalamlne (60:40, v/v); Flow rate - 1.2 ml/mln; Injection volume - 25 gl. The method was validated in terms of linearity, specificity, precision and accuracy. These all showed the proposed method was suitable and reliable for intended application.