Directly Purification of Ostruthin from Paramignya trimera roots by CPC (centrifugal partition chromatography)

Abstract

Ostruthin was successfully isolated from the extract of Paramignya trimera (Oliver) Burkill roots by centrifugal partition chromatography (CPC) in one run. The two-phase solvent system composed of n-hexane-ethyl acetate- methanol-water (2:1:2:1 v/v) in ascending mode (the upper organic phase was the mobile phase), with flow rate of 8.0 (mL/min) and rotation speed of 1600 rpm, was used. The purity of the ostruthin after isolation is 97.5 % confirmed by HPLC-DAD at 320 nm. The present study highlights the ability of a CPC system to obtain high purity ostruthin directly from the P. trimera roots ethyl acetate extract.