Chemical and pharmacological evaluation of Manglicolous lichen Graphis ajarekarii Patw. & c.R.Kulk.

Abstract

The chemical examination of ethanolic extract of Manglicolous lichen Graphis ajarekarii (Ga-Et) resulted in isolation of three known metabolites i.e. chiodectonic acid (l) graphenone (2) and graphisquinone (3). All the isolates (1-3) and Ga-Et were screened against DPPH, superoxide free radicals and ferric ion reducing power assays, and six different cancer cell lines (MDA-MB-231, SW620, HeLa, FADU, A549, SKOV3) and one normal human cell line (NHME). Compound 1 exhibited equivalent inhibition of superoxide free radical as that of the standard (ascorbic acid) with IC50 value of 32.0 µg/mL. Peering at the SRB assay results, it is observed that the better ICS50 values were determined by 3 of 15.5, 16.5 and 26.50 µg,/mL on SW620, FADU and MDA-MB-231, respectively. Addition to, all the tested samples showed low cell lysis on NHME indicate non-toxic. Consequently, the outcomes revealed that the G. ajarekarii is the new source to treat oxidative stress and cancer.