Synthesis and antibacterial activity of composite based on chitosan-grafted-(N-butyl acrylate) and silver nanoparticles

Abstract

It has been found for a long time that chitosan (CS) and silver nanopartieles (AgNPs) have outstanding antibacterial activities but there have been some drawbacks restricting a popular usage as such. Consequently, in this research, CS modified was combined with AgNPs with the purpose of expanding applications and enhancing the antibacterial activities. The colloid of CS and AgNPs (CS/Ag) was synthesized via chemical reduction while grafting copolymerization was carried out with monomer n-butyl acrylate (BA) and tert-butyl hydroperoxide (TBHP) as an initiator generating composite between CS-grafted-BA and AgNPs (CS-g-BA/Ag). The effects of synthesis parameters on the synthesizing CS-g-BA/Ag composite were studied by determining the grafting percentage (G%) and grafting efficiency (E%), The results revealed that the highest G 80 % and E 52 %, were obtained in the conditions as follows: the content of Ag = 0.5 %, BA/CS 4 w/w, TBHP/CS=7 mL/g with the concentration of TBHP aqueous solution is 20 mM, the concentration of CS is 0.85 % in acetic acid 0.6 and assessed by Fourier Transform Infrared spectroscopy, Ultraviolet-visible spectroscopy, Differential Scanning Calorimetry, Transmission Electron Microscope, Field Emission Scanning Electron Microscope and antibacterial activity.