Please use this identifier to cite or link to this item: https://dspace.ctu.edu.vn/jspui/handle/123456789/69646
Title: Isolation and selection of Bacillus strains with high potential probiotic that used in catfish farming (Pangasianodon hypophthalnius)
Authors: Nguyen, Van Minh
Nguyen, Hoang Tuan Duy
Do, Phuong Quynh
Tran, Thi A Ni
Tran, Kien Duc
Duong, Nhat Linh
Keywords: Bacillus subtilis
Probiotic
Edwardsiella ictaluri
Perionyx cxcavatus
Pangasianodon hypophthalmus
Issue Date: 2019
Series/Report no.: Journal of Science Ho Chi Minh Open University;Vol. 10, No. 01 .- P.3-12
Abstract: In this study, we isolated 28 strains of Bacillus spp. from water samples, catfish pond mud samples and earthworm manure (Perionyx excavates). By the cross-streak agar methods, 22 Bacillus strains showed the inhibition ability to Edwardsiella ictaluri, which caused Bacillary Necrosis Pangasius (BNP) in catfish (Pangasianodon hypophthalmus). Both Bacillus sp. Q16 and Q111 strains showed the highest inhibition to E. ictaluri by the double-layer agar methods. Finally, two Bacillus strains (Q16, Q111) were selected as a source of potential probiotic because of the ability of extracellular enzyme secretion (protease, amylase, cellulose) strong growth at 0,1-1% salt concentrations, survival within the pH range 6-8, resistance to low pH and low bile salts, inability to produce haemolysin enzyme, sensitivity to eight antibiotics in the three impacting groups (inhibition of wall synthesis, inhibition mechanism of protein synthesis, inhibition of nucleic acid synthesis). Two Bacillus strains (Q16, Q111) were identified that they belong to Bacillus subtilis by biochemical method and 16S rRNA gene sequencing method. This study indicated that two Bacillus strains (Q16, QIII) isolated from catfish pond can be applied as high potential probiotics that used to farm catfish.
URI: https://dspace.ctu.edu.vn/jspui/handle/123456789/69646
ISSN: 1859-3453
Appears in Collections:Khoa học Trường ĐH Mở Tp.HCM (Journal of Science HCM Open University)

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