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https://dspace.ctu.edu.vn/jspui/handle/123456789/71163
Title: | Expression and possible roles of extracellular signal-related kinases 1-2 (ERK1-2) in mouse primordial germ cell development |
Authors: | Sorrento, Maria Klinger, Francesca Gioia Iona, Saveria Rossi, Valerio Marcozzi, Serena Felici, Massimo De |
Keywords: | Extracellular signal-regulated kinases (ERKs) Gene expression Meiosis Oogenesis Primordial germ cells |
Issue Date: | 2020 |
Series/Report no.: | Journal of reproduction and development;Vol. 66, No. 05 .- P.399-409 |
Abstract: | In the present work, we described the expression and activity of extracellular signal-related kinases 1-2 (ERK1-2) in mouse primordial germ cells (PGCs) from 8.5-14.5 days post coitum (dpc) and investigated whether these kinases play a role in regulating the various processes of PGC development. Using immunofluorescence and immunoblotting to detect the active phosphorylated form of ERK1-2 (p-ERK1-2), we found that the kinases were present in most proliferating 8.5-10.5 dpc PGCs, low in 11.5 dpc PGCs, and progressively increasing between 12.5-14.5 dpc both in female and male PGCs. In vitro culture experiments showed that inhibiting activation of ERK1-2 with the MEK-specific inhibitor U0126 significantly reduced the growth of 8.5 dpc PGCs in culture but had little effect on 11.5-12.5 dpc PGCs. Moreover, we found that the inhibitor did not affect the adhesion of 11.5 dpc PGCs, but it significantly reduced their motility features onto a cell monolayer. Further, while the ability of female PGCs to begin meiosis was not significantly affected by U0126, their progression through meiotic prophase I was slowed down. Notably, the activity of ERK1 -2 was necessary for maintaining the correct expression of oocyte- specific genes crucial for germ cells survival and the formation of primordial follicles. |
URI: | https://dspace.ctu.edu.vn/jspui/handle/123456789/71163 |
ISSN: | 0916-8818 |
Appears in Collections: | The journal of reproduction and development |
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