Please use this identifier to cite or link to this item: https://dspace.ctu.edu.vn/jspui/handle/123456789/71163
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dc.contributor.authorSorrento, Maria-
dc.contributor.authorKlinger, Francesca Gioia-
dc.contributor.authorIona, Saveria-
dc.contributor.authorRossi, Valerio-
dc.contributor.authorMarcozzi, Serena-
dc.contributor.authorFelici, Massimo De-
dc.date.accessioned2021-12-22T09:09:18Z-
dc.date.available2021-12-22T09:09:18Z-
dc.date.issued2020-
dc.identifier.issn0916-8818-
dc.identifier.urihttps://dspace.ctu.edu.vn/jspui/handle/123456789/71163-
dc.description.abstractIn the present work, we described the expression and activity of extracellular signal-related kinases 1-2 (ERK1-2) in mouse primordial germ cells (PGCs) from 8.5-14.5 days post coitum (dpc) and investigated whether these kinases play a role in regulating the various processes of PGC development. Using immunofluorescence and immunoblotting to detect the active phosphorylated form of ERK1-2 (p-ERK1-2), we found that the kinases were present in most proliferating 8.5-10.5 dpc PGCs, low in 11.5 dpc PGCs, and progressively increasing between 12.5-14.5 dpc both in female and male PGCs. In vitro culture experiments showed that inhibiting activation of ERK1-2 with the MEK-specific inhibitor U0126 significantly reduced the growth of 8.5 dpc PGCs in culture but had little effect on 11.5-12.5 dpc PGCs. Moreover, we found that the inhibitor did not affect the adhesion of 11.5 dpc PGCs, but it significantly reduced their motility features onto a cell monolayer. Further, while the ability of female PGCs to begin meiosis was not significantly affected by U0126, their progression through meiotic prophase I was slowed down. Notably, the activity of ERK1 -2 was necessary for maintaining the correct expression of oocyte- specific genes crucial for germ cells survival and the formation of primordial follicles.vi_VN
dc.language.isoenvi_VN
dc.relation.ispartofseriesJournal of reproduction and development;Vol. 66, No. 05 .- P.399-409-
dc.subjectExtracellular signal-regulated kinases (ERKs)vi_VN
dc.subjectGene expressionvi_VN
dc.subjectMeiosisvi_VN
dc.subjectOogenesisvi_VN
dc.subjectPrimordial germ cellsvi_VN
dc.titleExpression and possible roles of extracellular signal-related kinases 1-2 (ERK1-2) in mouse primordial germ cell developmentvi_VN
dc.typeArticlevi_VN
Appears in Collections:The journal of reproduction and development

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