Vui lòng dùng định danh này để trích dẫn hoặc liên kết đến tài liệu này: https://dspace.ctu.edu.vn/jspui/handle/123456789/71182
Nhan đề: Transcriptional activation of the mouse Scd2 gene by interdependent enhancers and long noncoding RNAs in ovarian granulosa cells
Tác giả: Mayama, Shota
Hamazaki, Nobuhiko
Maruyama, Yuki
Bara, Shin Matsu
Kimura, Atsushi P.
Từ khoá: Granulosa cell
Histone acetylation
Interdependent enhancer
Long noncoding RNA
Ovary
Stearoyl-CoA desaturase
Năm xuất bản: 2020
Tùng thư/Số báo cáo: Journal of reproduction and development;Vol. 66, No. 05 .- P.435-444
Tóm tắt: Specific gene expression in granulosa cells is key for the function of ovary, but the molecular mechanism of transcriptional activation is not well studied. Here we investigated the regulatory mechanism of the mouse stearoyl-CoA desaturase 2 (Scd2) gene encoding an enzyme for lipid metabolism. Northern blot and in situ hybridization indicated that the mouse Scd2 mRNA was highly expressed in ovarian granulosa cells. We found four conserved noncoding sequences (CNSs) and two long noncoding RNAs (IncRNAs) transcribed from regions upstream of the Scd2 gene as candidates of regulatory elements/factors. These IncRNAs were predominantly transcribed in the opposite direction to Scd2 and localized in nuclei and showed the correlation with Scd2 expression, raising the possibility of their transcriptional regulatory roles. Indeed, knockdown of both IncRNAs, IncRNA-sc1 and lncRNA-sc2, significantly decreased the Scd2 mRNA level in primary granulosa cells. Then, we investigated the histone modification pattern at this locus by a chromatin immunoprecipitation assay, and two CNSs, CNS1 and CNS2, were found to be marked with high levels of histone H3K9/K27 acetylation in primary granulosa cells. By a reporter gene assay, both CNS1 and CNS2 interdependently exhibited enhancer activity for the Scd2 promoter in primary granulosa cells. These data suggest that the mouse Scd2 gene is activated by two IncRNAs and interdependent enhancers in ovarian granulosa cells, which provides a new insight into transcriptional activation in granulosa cells.
Định danh: https://dspace.ctu.edu.vn/jspui/handle/123456789/71182
ISSN: 0916-8818
Bộ sưu tập: The journal of reproduction and development

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