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https://dspace.ctu.edu.vn/jspui/handle/123456789/71204
Title: | RNA-binding protein Ptbp1 regulates alternative splicing and transcriptome in permatogonia and maintains spermatogenesis in concert with Nanos3 |
Authors: | Senoo, Manami Hozoji, Hiroshi Ishikawa-Yamauchi, Yu Takijiri, Takashi Ohta, Sho Ukai, Tomoyo Kabata, Mio Yamamoto, Takuya Yamada, Yasuhiro Ikawa, Masahito Ozawa, Manabu |
Keywords: | Alternative splicing PTBP1 RNA-binding protein Spermatogonia Spermatogenesis |
Issue Date: | 2020 |
Series/Report no.: | Journal of reproduction and development;Vol. 66, No. 05 .- P.459-467 |
Abstract: | PTBP1, a well-conserved RNA-binding protein, regulates cellular development by tuning posttranscriptional mRNA modification such as alternative splicing (AS) or mRNA stabilization. We previously revealed that the loss of Ptbp1 in spermatogonia causes the dysregulation of spermatogenesis, but the molecular mechanisms by which PTBP1 regulates spermatogonium homeostasis are unclear. In this study, changes of AS or transcriptome in Ptbp1-knockout (KO) germline stem cells (GSC), an in vitro model of proliferating spermatogonia, was determined by next generation sequencing. We identified more than 200 differentially expressed genes, as well as 85 genes with altered AS due to the loss of PTBP1. Surprisingly, no differentially expressed genes overlapped with different AS genes in Ptbp1-KO GSC. In addition, we observed that the mRNA expression of Nanos3, an essential gene for normal spermatogenesis, was significantly decreased in Ptbp1-KO spermatogonia. We also revealed that PTBP1 protein binds to Nanos3 mRNA in spermatogonia. Furthermore, Nanas3⁺ˡ⁻ ;Ptbp1⁺ˡ⁻ mice exhibited abnormal spermatogenesis, which resembled the effects of germ cell-specific Ptbp1 KO, whereas no significant abnormality was observed in mice heterozygous for either gene alone. These data implied that PTBP1 regulates alternative splicing and transcriptome in spermatogonia under dillerent molecular pathways, and contributes spermatogenesis, at least in part, in concert with NANOS3. |
URI: | https://dspace.ctu.edu.vn/jspui/handle/123456789/71204 |
ISSN: | 0916-8818 |
Appears in Collections: | The journal of reproduction and development |
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