Please use this identifier to cite or link to this item: https://dspace.ctu.edu.vn/jspui/handle/123456789/71205
Full metadata record
DC FieldValueLanguage
dc.contributor.authorWake, Yui-
dc.contributor.authorKaneko, Takehito-
dc.date.accessioned2021-12-23T03:05:28Z-
dc.date.available2021-12-23T03:05:28Z-
dc.date.issued2020-
dc.identifier.issn0916-8818-
dc.identifier.urihttps://dspace.ctu.edu.vn/jspui/handle/123456789/71205-
dc.description.abstractGenome editing technology contributes to the quick and highly efficient production of genetically engineered animals. These animals are helpful in clarifying the mechanism of human disease. Recently, a new electroporation technique (TAKE: Technique for animal knockout system by electroporation) was developed to produce genome-edited animals by- introducing nucleases into intact embryos using electroporation instead of the microinjection method. The aim of this study was to increase the efficiency of production of genome-edited animals using the TAKE method. In the conventional protocol, it was difficult to confirm the introduction of nucleases into embryos and energization during operation. Using only embryos that introduced nucleases for embryo transfer, it will lead to increased efficiency in the production of genome-edited animals.vi_VN
dc.language.isoenvi_VN
dc.relation.ispartofseriesJournal of reproduction and development;Vol. 66, No. 05 .- P.469-473-
dc.subjectCRISPR/Casvi_VN
dc.subjectElectroporationvi_VN
dc.subjectEmbryosvi_VN
dc.subjectGenome editingvi_VN
dc.subjectMousevi_VN
dc.titleProduction of genome-edited mice by visualization of nucleases introduced into the embryos using electroporationvi_VN
dc.typeArticlevi_VN
Appears in Collections:The journal of reproduction and development

Files in This Item:
File Description SizeFormat 
_file_
  Restricted Access
1.45 MBAdobe PDF
Your IP: 3.144.248.150


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.