Developmental and bone development indexes of medaka fish at 11 and 16 days of age

Abstract

The teleost fish medaka (Oryzias latipes) is currently used by laboratories worldwide as a model for many human diseases, including bone diseases. Our research group focuses on a medaka model for osteoporosis. To characterize the bone indexes of diseased animals, the bone development indexes of healthy wild-type fish are required for comparative analysis. Thus, this study examined the developmental and bone development indexes of wild-type medaka larvae at 11 and 16 days of age, the two developmental stages selected for the analysis of diseased patterns in osteoporosis-induced animals. The assessed parameters included the total body length of live larvae, number of caudal fin rays, vertebrae, and neural and hemal arches, as well as the total areas of mineralized vertebrae and lengths of mineralized arches. The obtained data are important for subsequent studies on bones using this fish model.