Analysis of genetic diversity in Pa Co pine (Pinus kwangtungensis Chun ex Tsiang) using RAPD and ISSR markers

Abstract

Pinas kwangtungensis (Pa Co pine) is one of three five-needle pine species in Vietnam, found on the slopes of limestone mountains at altitudes between 1200 and 1500 m. Global warming and long-term deforestation threaten the existence of the species in nature. The genetic diversity of plant populations provides a background for future conservation and improvement programmes. However, the genetic diversity of Pa Co pine is unknown. This study aimed to use inter-simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) genetic markers to evaluate the genetic-diversity parameters of P. kwangtungensis to understand the genetic effects of small and fragmented populations, as well as provide the genetic background for its conservation. The results from this study enhance the understanding of the genetic effects of small and fragmented populations of native species that are rare, vulnerable, and require conservation.