Optimization of culture conditions of iron-enriched biomass of Saccharomyces pastorianus by response surface methodology

Abstract

Yeast biomass enriched with iron is used in a profound and safe treatment for anaemia. In this work, response surface methodology (RSM) was used to survey the response of culture conditions (temperature, degrees brix, time, and initial iron concentration) to the bioaccumulation of ferric ion (Fe³⁺) in yeast (Saccharomycespastorianus). On the other hand, the Box-Behnken design was used to determine the optimum conditions of 24°C, 13°Bx of the culture solid content for 49 h incubation and 656 ppm of initial iron concentration. The total Fe³⁺ content in the biomass was significantly affected by the culture temperature and degrees brix (p<0.0001). Under optimum conditions, the maximum level of Fe (III) ions in the dry cell weight of S. pastorianus was 16.82±0.65 mg/g. The results from statistical analysis showed that the model was significant (p<0.0001) and adequate.