Vui lòng dùng định danh này để trích dẫn hoặc liên kết đến tài liệu này: https://dspace.ctu.edu.vn/jspui/handle/123456789/71735
Nhan đề: Phleomycin resistance gene as a reliable selection marker for Agrobacterium tumefaciens-mediated transformation of the citrus postharvest pathogen Penicillium digitatum
Tác giả: Tran, Van-Tuan
Vu, Tao Xuan
Từ khoá: Asrobacterium-mediated transformation
Citrus postharvest pathogen
DsRed fluorescent reporter gene
Penicillium digitatum
Phleomycin resistance marker
Năm xuất bản: 2020
Tùng thư/Số báo cáo: Vietnam Journal of Science, Technology and Engineering;Vol. 62, No. 02 .- P.83-88
Tóm tắt: Penicillium digitatum exists in nature as a causative agent of green mould disease in citrus fruits at the postharvest stages. Inspections of the molecular mechanism of the host invasion by P. digitatum usually require suitable selection markers for genetic manipulation. In this study, we recruited the phleomycin resistance gene as a selection marker for the genetic transformation of P. digitatum using Agrobacterium tumefaciens. The results showed that the growth of the wild strain P. digitatum PdVN1 from fungal spores is quite sensitive to phleomycin and is inhibited at a concentration of 50 µg/ml, whereas growth from fungal mycelium is more tolerant and completely suppressed at a concentration of 200 µg/ml. Under optimised conditions, the A. tumefaciens-mediated transformation (ATMT) efficiency of P. digitatum with the phleomycin resistance marker could reach over 1000 transformants per 10⁶ spores. All the tested transformants presented the integration of T-DNA in their genomes and were mitotically stable for the phleomycin resistance. Furthermore, the results also revealed the success for heterologous expression of the DsRed fluorescent gene in the fungus, in which the strong red fluorescent signal could be observed over the whole fungal mycelium and spores. Our work demonstrates for the first time that the phleomycin resistance gene can serve as a reliable selection marker for A. tumefaciens-mediated transformation of the postharvest pathogen P. digitatum. This selection marker can be exploited for T-DNA insertional mutagenesis and for functional investigations of target genes involved in citrus decay by P. digitatum.
Định danh: https://dspace.ctu.edu.vn/jspui/handle/123456789/71735
ISSN: 2525-2461
Bộ sưu tập: Vietnam journal of Science, Technology and Engineering

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