Vui lòng dùng định danh này để trích dẫn hoặc liên kết đến tài liệu này: https://dspace.ctu.edu.vn/jspui/handle/123456789/71747
Nhan đề: Development of inducer-free expression plasmids using IPTG-inducible Pspac promoter for Bacillus subtilis
Tác giả: Chu, Thi Bich Phuong
Phan, Hanh Thi Thu
Nguyen, Hoang Duc
Phan, Trang Thi Phuong
Từ khoá: Bacillus subtilis
Inducer-free
pHT vector
Pspac
Weak promoter
Năm xuất bản: 2021
Tùng thư/Số báo cáo: Vietnam Journal of Science, Technology and Engineering;Vol. 63, No. 01 .- P.64-70
Tóm tắt: An inducer-free expression vector for the low expression levels in Bacillus subtilis (B. subtilis) is necessary for fundamental research. In this study, we constructed inducer-free expression plasmids carrying Pspac, a well-known IPTG-inducible promoter, by removing a part of the lad gene. Then, we analysed the expression of the target genes bgaB and gfp* in B. subtilis. Western blot experiments demonstrated that the reporters from the inducer-free plasmids with Pspac could be produced at low levels in B. subtilis strains and were equivalent to their corresponding inducible constructs with 1 mM IPTG. The reporter activities showed that inducer-free expression from the Pspac promoter was dramatically less than that of the inducer-free plasmids with the strong promoters Pgrac01 and Pg/m100 reported previously, about 16.2 to 20.3 times for BgaB and 24.7 to 34.3 times for GFP⁺, respectively. In conclusion, the inducer-free expression vectors carrying Pspac promoters allow the constitutive expression of heterologous recombinant proteins at low levels in B. subtilis.
Định danh: https://dspace.ctu.edu.vn/jspui/handle/123456789/71747
ISSN: 2525-2461
Bộ sưu tập: Vietnam journal of Science, Technology and Engineering

Các tập tin trong tài liệu này:
Tập tin Mô tả Kích thước Định dạng  
_file_
  Giới hạn truy cập
2.67 MBAdobe PDF
Your IP: 3.138.134.106


Khi sử dụng các tài liệu trong Thư viện số phải tuân thủ Luật bản quyền.