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DC Field | Value | Language |
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dc.contributor.author | Chu, Thi Bich Phuong | - |
dc.contributor.author | Phan, Hanh Thi Thu | - |
dc.contributor.author | Nguyen, Hoang Duc | - |
dc.contributor.author | Phan, Trang Thi Phuong | - |
dc.date.accessioned | 2021-12-30T08:43:44Z | - |
dc.date.available | 2021-12-30T08:43:44Z | - |
dc.date.issued | 2021 | - |
dc.identifier.issn | 2525-2461 | - |
dc.identifier.uri | https://dspace.ctu.edu.vn/jspui/handle/123456789/71747 | - |
dc.description.abstract | An inducer-free expression vector for the low expression levels in Bacillus subtilis (B. subtilis) is necessary for fundamental research. In this study, we constructed inducer-free expression plasmids carrying Pspac, a well-known IPTG-inducible promoter, by removing a part of the lad gene. Then, we analysed the expression of the target genes bgaB and gfp* in B. subtilis. Western blot experiments demonstrated that the reporters from the inducer-free plasmids with Pspac could be produced at low levels in B. subtilis strains and were equivalent to their corresponding inducible constructs with 1 mM IPTG. The reporter activities showed that inducer-free expression from the Pspac promoter was dramatically less than that of the inducer-free plasmids with the strong promoters Pgrac01 and Pg/m100 reported previously, about 16.2 to 20.3 times for BgaB and 24.7 to 34.3 times for GFP⁺, respectively. In conclusion, the inducer-free expression vectors carrying Pspac promoters allow the constitutive expression of heterologous recombinant proteins at low levels in B. subtilis. | vi_VN |
dc.language.iso | en | vi_VN |
dc.relation.ispartofseries | Vietnam Journal of Science, Technology and Engineering;Vol. 63, No. 01 .- P.64-70 | - |
dc.subject | Bacillus subtilis | vi_VN |
dc.subject | Inducer-free | vi_VN |
dc.subject | pHT vector | vi_VN |
dc.subject | Pspac | vi_VN |
dc.subject | Weak promoter | vi_VN |
dc.title | Development of inducer-free expression plasmids using IPTG-inducible Pspac promoter for Bacillus subtilis | vi_VN |
dc.type | Article | vi_VN |
Appears in Collections: | Vietnam journal of Science, Technology and Engineering |
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