Please use this identifier to cite or link to this item: https://dspace.ctu.edu.vn/jspui/handle/123456789/39511
Title: Isolation and identification of endophytic bacteria associated with Bruguiera sexagula and Ceriops decandra in mangrove forest of Soc Trang province, Vietnam
Authors: Ho, Thanh Tam
Tran, Vu Phuong
Cao, Ngoc Diep
Keywords: 16S rRNA gene sequence
Bruguiera sexagula
Ceriops decandra
Endophytic bacteria
Nitrogen fixation
Phosphate solubilization
Issue Date: 2019
Series/Report no.: International Journal of Innovations in Engineering and Technology (IJIET);Vol. 14 No. 01 .- P.50-61
Abstract: Total of 68 bacterial isolates inside the roots of Bruguiera and Ceriops in mangrove forest of Soc Trang province, Vietnam. They were isolated from 16 root samples on the two kinds of Burk’ N-free and NBRIP semi-solid medium with the presence of pellice located below the surface of medium 1-2 mm as indicator. Almost their colonies have round-shaped; milky, white clear (on Burk’s medium) and yellow (on NBRIP medium); entire or lobate margin; diameter size of these colonies varied from 0.2 to 3.0 mm and all of them are Gram-negative by Gram stain, motility. 38/68 isolates grew on NBRIP medium and 30/68 isolates grew on Burk’s N-free medium and all isolates did not produce siderophore. From 68 isolates, screening of 6 isolates had high nitrogen fixation ability, 8 isolates insolved high phosphate and 9 isolates biosynthezed high IAA concentration in NBRIP tryptophan-free liquid medium; in Burk’s N-free without tryptophan medium, selecting of 11 isolates had high phosphate solubilization ability and 8 isolates biosynthezed high IAA concentration. Nine good isolates were selected to identify, sequencing and compared with bacterial 16s-rRNA genes in Genbank using BLAST N software. The results showed that 9 strains belonged to 7 genera as Mangrovibacter, Acinetobacter, Gallaecimonas, Martelella, Stenotrophomonas, Curtobacterium and Defluviimonas with 99% similarity.
URI: https://dspace.ctu.edu.vn/jspui/handle/123456789/39511
ISSN: 2319-1058
Appears in Collections:Tạp chí quốc tế

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