Please use this identifier to cite or link to this item: https://dspace.ctu.edu.vn/jspui/handle/123456789/4200
Title: Influence of leptin genotypes on milk fat and protein content of crossbred Holstein Friesian x lai Sind cows
Authors: Nguyễn, Trọng Ngữ
Le, Thuy Bao Quynh
Nguyễn, Văn Hớn
Nguyễn, Thị Hồng Nhân
Đỗ, Võ Anh Khoa
Lâm, Thái Hùng
Nguyễn, Hồng Xuân
Keywords: Cow
Leptin gene polymorphism
Milk quality trait
Issue Date: 2015
Series/Report no.: The Journal of Animal & Plant Sciences;25 .- p.304-308
Abstract: The aim of this study was to investigate leptin (LEP) gene polymorphisms in crossbred Holstein Friesian (HF) x Lai Sind cattle and their effects on milk yield and milk quality traits. Milk yield was recorded for lactation period of 305 days. Milk fat and protein were estimated using the Milko Tester machine. DNA extracted from blood samples (n=206) was amplified by PCR technique followed by sequencing for single nucleotide polymorphism (SNP) identification. Applied genotyping for the two SNPs (g.C1180T and g.C2059T) was done by PCR-RFLP method with the presence of Kpn2I and Sau3AI restriction enzymes, respectively. At the LEP/Kpn2I locus, cows of CT genotype accounted for the highest proportion (78%) whereas at the second SNP, the frequency of CC dominated (63%). In the association analysis, the LEP/Kpn2I polymorphism did not affect 305-day milk yield, but showed significant influence on milk quality traits, of which cows with CT genotype had higher protein (3.67%) and fat (3.99%) content than ones with CC and TT. The other locus (LEP/Sau3AI) was not associated with any traits analyzed. In conclusion, the g.C1180T SNP could be included in marker assisted selection of crossbred HF cows for improving protein and fat percentages.
URI: http://dspace.ctu.edu.vn/jspui/handle/123456789/4200
ISSN: 1018-7081
Appears in Collections:Tạp chí quốc tế

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