Please use this identifier to cite or link to this item: https://dspace.ctu.edu.vn/jspui/handle/123456789/43156
Title: Cloning and expression of maltooligosyltrehalose trehalohydrolase from sulfolobus solfataricus dsm 1616 in bacillus subtilis wb800
Authors: Nguyen, Tien Cuong
Nguyen, Thi Hien Trang
Nguyen, Thi Thao
Le, Thanh Hoang
Nguyen, Sy Le Thanh
Nguyen, Thi Anh Tuyet
Nguyen, Manh Dat
Le, Duc Manh
Do, Thi Thanh Huyen
Nguyen, Thi Thu
Nguyen, Thi Trung
Hoang, Thi Yen
Do, Thi Tuyen
Keywords: Cloning
Expression
Maltooligosyltrehalose trehalohydrolase
Sulfobolus solfataricus DSM 1616
Bacillus subtilis WB800
Issue Date: 2020
Series/Report no.: Journal of Biotechnology;Vol. 18, No. 02 .- P.363-372
Abstract: Maltooligosyltrehalose trehalohydrolase (MTHase) is an industrial enzyme for the production of trehalose. A DNA fragment of 1680 bp encoding for MTHase was cloned from Sulfoholus solfataricus DSM 1616 then fused with promoter acoA-amyE already amplified from pMSE3 vector by PCR to generate an expression cassette acoMTH. Afterward the cassette was inserted into pAC7 vector for expression of the gene in Bacillus subtilis WB800 - a conventional expression system.
URI: https://dspace.ctu.edu.vn/jspui/handle/123456789/43156
ISSN: 1811-4989
Appears in Collections:Công nghệ sinh học

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