Please use this identifier to cite or link to this item: https://dspace.ctu.edu.vn/jspui/handle/123456789/54715
Title: Nghiên cứu phân lập, nhận dạng cleistantoxin từ quả cây chà chôi (Cleistanthus tonkinensis Jabl., Euphorbiaceae) làm nguyên liệu thiết lập chất chuẩn
Authors: Nguyễn, Lâm Hồng
Đào, Tú Anh
Lê, Công Vinh
Phạm, Văn Cường
Đoàn, Thị Mai Hương
Trần, Việt Hùng
Keywords: Cleistanthus tonkinensis Jabl
Isolation
Identification
Purity determination
Cleistantoxin
Issue Date: 2018
Series/Report no.: Tạp chí Dược học;Số 512 .- Tr.21-24
Abstract: On continuance of the previous programmed screening to reveal that ethyl acetate extracts from the fruits of the 2 Cleistanthus species (C. indochinensls Merr. ex Croiz and C. tonkinensis Jab!., Euphorbiaceae) possessing a strong cytotoxicity against KB cells (88.4 and 95.2 % inhibition at 1 µg/mL), cleistantoxin and some aryltetralin lignans were isolated from these species. The obtained cleistantoxin was identified physically by appearance, melting range (199 - 202 °C), specific rotation ([α]D²⁰= -133,400), and spectrometrically by 1D-NMR, 2D-NMR, HR-MS, CD, IR, UV, etc. As the major active component of the plant, Cleistantoxin proved to have a significant cytotoxicity against the following cancer cell lines: MCF-7 (IC₅₀ = 0.036 µM), MCF-7R (ICX= 0.014 µM) and HT29 (IC₅₀= 0.035 µM). In addition, cleistantoxin showed structural similarity to etoposide which is currently used for lung cancer treatment. To establish a primary chemical reference substance (PCRS). 4.023 g cleistantoxin was purified (>99.0% by HPLC/DAD analysis), meeting the quality and purity requirments of the International Pharmacopoeia (IP) and World Health Organization (WHO) guide.
URI: https://dspace.ctu.edu.vn/jspui/handle/123456789/54715
ISSN: 0866-7861
Appears in Collections:Dược học

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